How can contaminants be extracted from water




















Official websites use. Share sensitive information only on official, secure websites. JavaScript appears to be disabled on this computer. Please click here to see any active alerts. Protection of our water resources is compromised by shortcomings in our abilities to adequately determine and reduce the full range of human and ecological health risks posed by waterborne contaminants, including chemicals and microbial pathogens.

EPA researchers are developing and evaluating effective tools to measure waterborne contaminants, which are essential for determining the risks these pollutants pose to people and the environment. With these tools, the source and level of contaminants can be evaluated, the risk posed to exposed communities can be characterized, and approaches for improving affected water resources can be developed. A key responsibility of the EPA under the Safe Drinking Water Act and the Clean Water Act is to develop methods to detect and quantitate waterborne contaminants, including microbes, that affect water systems.

Recent efforts in method development and application have focused on opportunistic pathogens, such as Legionella and Mycobatcteria , which are of particular concern for drinking water systems.

This work has provided more detail on the frequency and level of contamination of these microbes in commercial and residential water systems. In addition, methods for detecting several enteric viruses were used in a large virus occurrence study, and a comparison of staining kits used for detecting the protozoan pathogen Giardia in source water was conducted.

Advancements are also being made toward the development of rapid, nucleic acid based methods to detect somatic coliphages, which are alternative indicators of fecal pollution and an attractive surrogate for viral pathogens. These methods have the potential to provide evidence of fecal contamination in a matter of hours instead of days.

Developing and using methods and models to measure chemical contaminants is a critical responsibility of the Agency. One recent area of focus has been the development and use of occurrence methods to detect several different classes of contaminants of emerging concern CECs , including per- and polyfluoroalkyl substances PFAS and algal toxins.

Collectively this work has helped characterize the level of chemical contamination across the Nation. Tools that enable EPA to measure the impact of contaminants on human health and the environment are essential for determining the risk posed by pollutants. Recent efforts have focused on the use of novel bioassays, such as those based on Adverse Outcome Pathways AOPs or metabolomic techniques, to assess the risk posed by various chemical pollutants.

Chromium III is a nutritionally essential element. Chromium VI is much more toxic than Chromium III and causes liver and kidney damage, internal hemorrhaging, respiratory damage, dermatitis, and ulcers on the skin at high concentrations. Copper Enters environment from metal plating, industrial and domestic waste, mining, and mineral leaching.

Can cause stomach and intestinal distress, liver and kidney damage, anemia in high doses. Imparts an adverse taste and significant staining to clothes and fixtures. Essential trace element but toxic to plants and algae at moderate levels. Cyanide Often used in electroplating, steel processing, plastics, synthetic fabrics, and fertilizer production; also from improper waste disposal.

Poisoning is the result of damage to spleen, brain, and liver. Dissolved solids Occur naturally but also enters environment from man-made sources such as landfill leachate, feedlots, or sewage. A measure of the dissolved "salts" or minerals in the water. May also include some dissolved organic compounds.

May have an influence on the acceptability of water in general. May be indicative of the presence of excess concentrations of specific substances not included in the Safe Water Drinking Act, which would make water objectionable. High concentrations of dissolved solids shorten the life of hot water heaters. Fluoride Occurs naturally or as an additive to municipal water supplies; widely used in industry. Decreases incidence of tooth decay but high levels can stain or mottle teeth.

Causes crippling bone disorder calcification of the bones and joints at very high levels. Hardness Result of metallic ions dissolved in the water; reported as concentration of calcium carbonate.

Calcium carbonate is derived from dissolved limestone or discharges from operating or abandoned mines. Decreases the lather formation of soap and increases scale formation in hot-water heaters and low-pressure boilers at high levels.

Iron Occurs naturally as a mineral from sediment and rocks or from mining, industrial waste, and corroding metal. Imparts a bitter astringent taste to water and a brownish color to laundered clothing and plumbing fixtures. Lead Enters environment from industry, mining, plumbing, gasoline, coal, and as a water additive. Affects red blood cell chemistry; delays normal physical and mental development in babies and young children.

Causes slight deficits in attention span, hearing, and learning in children. Can cause slight increase in blood pressure in some adults. Probable carcinogen. Manganese Occurs naturally as a mineral from sediment and rocks or from mining and industrial waste. Causes aesthetic and economic damage, and imparts brownish stains to laundry. Affects taste of water, and causes dark brown or black stains on plumbing fixtures.

Relatively non-toxic to animals but toxic to plants at high levels. Mercury Occurs as an inorganic salt and as organic mercury compounds.

Enters the environment from industrial waste, mining, pesticides, coal, electrical equipment batteries, lamps, switches , smelting, and fossil-fuel combustion. Causes acute and chronic toxicity. Targets the kidneys and can cause nervous system disorders.

Nickel Occurs naturally in soils, groundwater, and surface water. Often used in electroplating, stainless steel and alloy products, mining, and refining. Damages the heart and liver of laboratory animals exposed to large amounts over their lifetime. Nitrate as nitrogen Occurs naturally in mineral deposits, soils, seawater, freshwater systems, the atmosphere, and biota.

More stable form of combined nitrogen in oxygenated water. Found in the highest levels in groundwater under extensively developed areas. Enters the environment from fertilizer, feedlots, and sewage. Toxicity results from the body's natural breakdown of nitrate to nitrite.

Causes "bluebaby disease," or methemoglobinemia, which threatens oxygen-carrying capacity of the blood. Selenium Enters environment from naturally occurring geologic sources, sulfur, and coal. Causes acute and chronic toxic effects in animals--"blind staggers" in cattle.

Nutritionally essential element at low doses but toxic at high doses. Silver Enters environment from ore mining and processing, product fabrication, and disposal. Often used in photography, electric and electronic equipment, sterling and electroplating, alloy, and solder. Because of great economic value of silver, recovery practices are typically used to minimize loss. Can cause argyria, a blue-gray coloration of the skin, mucous membranes, eyes, and organs in humans and animals with chronic exposure.

Sodium Derived geologically from leaching of surface and underground deposits of salt and decomposition of various minerals. Human activities contribute through de-icing and washing products. Can be a health risk factor for those individuals on a low-sodium diet. Yokoyama K. Our recent experiences with sarin poisoning cases in Japan and pesticides users with references to some selected chemicals. Neuro Toxicol. Gravriel A. Incidence of mesophilic Aeromonas within a public drinking water supply in north-east Scotland.

Kivanc M. The occurrence of Aeromonas in drinking water, tap water and the Porsuk River. Water Sentinel System Architecture. Water Research Foundation. Comparison of multivariate classification methods for contamination event detection in water distribution systems. Procedia Eng. Liu S. A method of detecting contamination events using multiple conventional water quality sensors.

Hou D. Detection of water-quality contamination events based on multi-sensor fusion using an extended Dempster—Shafer method. Wen J. Genetic algorithm based fuzzy comprehensive evaluation of water quality in Dongzhaigang. Murray R. Handbook of Water and Wastewater Systems Protection.

Real-world case studies for sensor network design of drinking water contamination warning systems; pp. Antony R. Detection principles and development of microfluidic sensors in the last decades. Liu K. Microfluidic systems for bio sensing. Wei Q. Detection and spatial mapping of mercury contamination in water samples using smart-phone. ACS Nano. Freire S. Interface between microfluidics and mass spectrometry. In: Li Dongqing.

Encyclopedia of Microfluidics and Nanofluidics. Springer US; Gao D. Recent advances in microfluidics combined with mass spectrometry: technologies and applications.

Chrimes A. Microfluidics and Raman microscopy: current applications and future challenges. Samiei E. Integration of biosensors into digital microfluidics: impact of hydrophilic surface of biosensors on droplet manipulation. Commission Directive. Sacher F. State-of-the-art in drinking water monitoring. In: van den Hoven T. Grasso G. Enumeration of Escherichia coli and coliforms in surface water by multiple tube fermentation and membrane filter methods.

Douterelo I. Methodological approaches for studying the microbial ecology of drinking water distribution systems. Eckner K. Comparison of membrane filtration and multiple-tube fermentation by the colilert and enterolert methods for the detection of waterborne coliform bacteria, Escherichia coli and Enterococci used in drinking and bathing water quality monitoring in Southern Sweden.

Omezuruike O. Microbiological and physicochemical analysis of different water samples used for domestic purposes in Abeokuta and Ojota, Lagos State, Nigeria, African.

Kimani-Murage E. Quality of water the slum dwellers use: the case of a Kenyan Slum. Urban Health. Brandao L. Diversity and antifungal susceptibility of yeasts isolated by multiple-tube fermentation from three freshwater lakes in Brazil.

Water Health. Mitchell, W. Curtis T. Influence of pH, oxygen and humic substances on ability of sunlight to damage fecal coliforms in waste pond water. Williams C. Water quality and fecal coliform levels in Georgia Oxbow Lakes relative to connectivity with Savannah River. Georgia J. Bartram J. Microbiological analyses.

In: Bratram J. Clescerl L. Standard Methods for Examination of Water and Wastewater. American Public Health Association, Amer. Koster W. Analytical methods for microbiological water quality testing; pp. Saati A. Environmental prevalence of pathogens in different drinking water resources in Makkah City. World Environ. Sobsey M. A simple membrane filter method to concentrate and enumerate male-specific RNA coliphages.

Grabow, A. Very, M. Uys, J. De Villiers. Research, Commission, Pretoria. Seeley N. The isolation of bacteriophages from the environment. Operational Control of Coagulation and Filtration Processes. Particle and natural organic matter removal in drinking water; pp. Zidek Z. Microfiltration method of removal bacterial contaminant and their monitoring by nitric oxide and Limulus assays. Nitric Oxide. Xiong B. Fouling of microfiltration membranes by flowback and produced waters from the Marcellus shale gas play.

Santos C. Clarification of red beet stalks extract by microfiltration combined with ultrafiltration. Food Eng. Van Hamme J. Recent advances in petroleum microbiology. Bouwer E. Removal of trace chlorinated organic compounds by activated carbon and fixed film bacteria. Teitzel G. Heavy metal resistance of biofilm and planktonic Psedomonas aeruginosa. Adan J. Bacterial inactivation and degradation of organic molecules by titanium dioxide supported on porous stainless steel photo catalytic membranes.

Heijnen L. Quantitative detection of E. Fratamico P. Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O, O, O and O in ground beef.

Foodborne Pathog. Snyder S. Role of membranes and activated carbon in the removal of endocrine disruptors and pharmaceuticals. Mullis K. The unusual origin of the polymerase chain reaction PCR Sci. Strachan T. Wiley-Liss; New York: Human Molecular Genetics. Demidov V. Horizon Bioscience; United Kingdom: Wittwer C. Magic in solution: an introduction and brief history of PCR.

In: Kennedy S. Bonetta S. Park S. Multiplex PCR assay for the detection and quantification of Campylobacter spp. FEMS Microbiol. Imtiaz J. WIT Press; Development of PCR protocol for the detection of escherichia coli in drinking water. Yu-Li T. Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction. Wahlfors J. Simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis by a rapid PCR method.

Braker G. Muyzer G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Kawai M. Fakruddin M. Ward L. Conventional PCR. In: Boonham N. Crown Copyright; Nucleic acid amplification: alternative methods of polymerase chain reaction. Bustin S. Sandhya S. Molecular beacons: a real-time polymerase chain reaction assay for the detecting Escherichia coli from fresh produce and water. Abia A. Occurrence of diarrhoeagenic Escherichia coli virulence genes in water and bed sediments of a river used by communities in Gauteng, South Africa.

E nviron. Sen K. Development of a sensitive detection method for stressed E. Lai X. Rapid identification and characterization of Francisella by molecular biology and other techniques. Open Microbiol. Janse I. Reliable detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis by using multiplex qPCR including internal controls for nucleic acid extraction and amplification. BMC Microbiol. Robinson B. Rapid, sensitive and discriminating identification of Naegleria spp. Rasmussen J.

Use of DNA melting simulation software for in silicon diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes. BMC Bioinf. Mull B. Improved method for the detection and quantification of naegleria fowleri in water and sediment using immunomagnetic separation and real-time PCR.

Parasitology Res. Bengtsson, M. Kubista, A. Stahlberg, L. Stroembom, inventors; Roche Molecular Systems Inc. Weis J. Trends Genet. Sheridan G. Liu L. Detection of Zaire Ebola virus by real-time reverse transcription-polymerase chain reaction. Ueda S. Identification of Cereulide-producing Bacillus cereus by nucleic acid chromatography and reverse transcription real-time PCR.

Biocontrol Sci. Effect of different heat treatments on the degradation of Salmonella nucleic acid. Food Saf. Izzo M. Comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf faeces in Australia. Wang X. Ultrasensitive detection of bacteria by targeting abundant transcripts.

Tomlinson J. Njir Z. Loop-mediated isothermal amplification lamp method for rapid detection of Trypanosoma brucei rhodesiense. PLoS Negl. Gibson W. The human serum resistance associated gene is ubiquitous and conserved in Trypanosoma brucei rhodesiense throughout East Africa. Welburn S. Identification of human-infective trypanosomes in animal reservoir of sleeping sickness in Uganda by means of serum-resistance-associated SRA gene. Njiru Z. Detection of Trypanosoma brucei rhodesiense in animals from sleeping sickness foci in East Africa using the serum resistance associated SRA gene.

Acta Trop. Truc P. Multilocus isoenzyme identification of Trypanosoma brucei stocks isolated in central Africa: evidence for an animal reservoir of sleeping sickness in Congo. Nagamine K. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Cell Probes. Zhen Z. Food Res. Notomi T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. Dinh D. An updated loop-mediated isothermal amplification method for rapid diagnosis of H5N1 Avian influenza viruses.

Kong Q. Parasites Vectors. Uemura N. Development of a loop-mediated isothermal amplification method for diagnosing Pneumocystis pneumonia. Parida M. Loop mediated isothermal amplification LAMP : A new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Kaneko H. Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. Wang F. A pilot study of quantitative loop-mediated isothermal amplification guided target theraphies for hospital-acquired pneumonia.

Yoshida A. Loop-mediated isothermal amplification method for rapid detection of the Periodontopathic Bacteria Porphyromonas gingivalis , Tannerella forsythia , and Treponema denticola. Sotiriadou I. Evaluation of loop-mediated isothermal amplification for detection of Toxoplasma gondii in water samples and comparative findings by polymerase chain reaction and immunofluorescence test IFT Diagn.

Mahittikorn A. Gallas-Lindemann C. Giardia and Cryptosporidium spp. Caipang C. Dauphin L. Comparative evaluation of automated and manual commercial DNA extraction methods for detection of Francisella tularensis DNA from suspensions and spiked swabs by real-time polymerase chain reaction. Versage J. Development of a multitarget real-time TaqMan PCR assay for enhanced detection of Francisella tularensis in complex specimens.

Broekhuijsen M. Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F. Kanitkar Y.

Fong T. Enteric viruses of humans and animals in aquatic environments: health risks, detection and potential water quality assessment tools. Gerba C. Assessment of enteric pathogen shedding during recreational activity and its impact on water quality.

Yadav R. Trends and perspectives of biosensors for food and environmental virology. Food Environ. Kim J. Quantitative real-time PCR approaches for microbial community studies in wastewater treatment systems: applications and considerations. Huang W. Water Sci. Jiang S. Real-time quantitative PCR for enteric adenovirus serotype 40 in environmental waters. Hmaied F. Comparison of two concentration methods for the molecular detection of enteroviruses in raw and treated sewage. Mashooq M. Development and evaluation of probe based real time loop mediated isothermal amplification for Salmonella : A new tool for DNA quantification.

Kliot A. Fluorescent in situ hybridization for the localization of viruses, bacteria and other microorganisms in insect and plant tissues.

Schimak M. MiL-FISH: Multilabeled oligonucleotides for fluorescence in situ hybridization improve visualization of bacterial cells.

Araya R. Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analysis. In: Haggblom M. Aerobic bacteria can only convert compounds when plenty of oxygen is present, because they need it to perform any kind of chemical conversion.

Usually the products they convert the contaminants to are carbon dioxide and water. When bacteria are used for water purification there are two sorts of conversion; one of these is anaerobic transfer. This means, that bacteria that are NOT oxygen dependent are converting the contaminants in the water. Anaerobic bacteria can only convert when oxygen levels are low, because they use other sorts of substances to perform chemical conversion. Anaerobic bacteria do not just develop carbon dioxide and water during conversion, but also methane gas.

This can be used to keep the machinery that supports the purification going. The anaerobic conversion of a substance requires more steps than aerobic conversion, but the final result is often less satisfactory. After anaerobic conversion usually aerobic bacteria bacteria that do use oxygen still need to finish the process, because the water is not clean enough yet.

Fertilizers such as phosphate are removed through addition of another chemical, usually iron. The substances than become solid precipitates, that can be filtered from the water. The removal of ammonium and nitrates is a little bit more complicated; it is a purification process that takes both aerobic and anaerobic conversion to remove them.

In the aerobic conversion stage there are two bacterial species involved. Nitrosomonas bacteria that convert ammonia to nitrite and Nitrobacter bacteria that convert nitrite to nitrate after that.

Although nitrate does not represent a direct health threat to most fish, high levels are still undesirable.



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